Quantitative Detection of Cucumber Mosaic Cucumovirus RNA with Microplate Hybridization Using Digoxigenin-Labeled Oligo-Deoxyribonucleotide Probe
نویسندگان
چکیده
منابع مشابه
Biolistic inoculation of gladiolus with cucumber mosaic cucumovirus.
A new method of inoculation of gladiolus with cucumber mosaic virus (CMV) was developed using the Bio-Rad Helios Gene Gun System. This method circumvents the traditional use of aphids to transmit CMV, a virus that is mechanically transmissible to many plant species but only with difficulty to gladiolus. Cartridges containing virus-coated gold microcarriers were prepared and the virus shot into ...
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CONTENTS Abstract We describe the use of PCR to generate sense and antisense template DNA for nonradioactive in vitro transcription reactions. The resulting digoxigenin-labeled RNA probes can be used in a small-scale Northern hybridization procedure that is performed in microcentrifuge tubes. This kind of Northern approach is especially useful when the expression of several genes is to be initi...
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ژورنال
عنوان ژورنال: Journal of the Faculty of Agriculture, Kyushu University
سال: 1999
ISSN: 0023-6152
DOI: 10.5109/24279